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. 2020 Jun 1;11:2729. doi: 10.1038/s41467-020-16575-0

Fig. 2. The FluoReSyn is rapidly degraded by the proteasome machinery.

Fig. 2

a Equally scaled, representative epifluorescence images of MG132 treated and untreated Reporter-cells which were fixed 2, 4, 8, or 16 h post treatment. The FluoReSyn accumulates in response to prolonged MG132 treatment of Reporter-cells as revealed by the increasing nuclear EGFP intensity. Reporter-cells untreated with MG132 yielded virtually no EGFP signal even after 16 h. Scale bar represents 20 µm. b Quantitative analyses of FluoReSyn signal (EGFP) in arbitrary units (a.u.) when Reporter-cells were treated or untreated with MG132. Per replication and condition more than 540 cells were analyzed. c FluoReSyn signal normalized by mCherry signal (EGFP to mCherry signal intensity). Error bars represent the SEM from three independent experiments (n = 3). Source data is available as a Source data file.