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. 2020 Jun 1;11:2729. doi: 10.1038/s41467-020-16575-0

Fig. 3. in vivo FluoReSyn activity in neurons of larval Xenopus laevis.

Fig. 3

a Organization of the Xenopus laevis olfactory system: olfactory receptor neurons (ORNs; red) residing in the olfactory epithelium project their axons to the olfactory bulb, where they synapse onto the second order projection neurons (gray). b Schematic of plasmid electroporation into the olfactory mucosa of anaesthetized Xenopus laevis tadpoles. c, e, g Left nostrils of three animals (A1–A3) were co-electroporated with FluoReSyn- and hαSyn-expressing plasmid. Scale bar represents 100 µm. d, f, h Respective right nostrils electroporated only with FluoReSyn. For all animals and nostrils identical plasmid concentrations and imaging settings were used. Basal and apical delineations of olfactory epithelium are indicated by line and dashed line, respectively. i Distribution of FluoReSyn mean fluorescence intensity of positive nuclei from the left nostrils (FluoReSyn and hαSyn plasmid; green dots) and nuclei from the right nostrils (FluoReSyn plasmid only; black dots) in arbitrary units (a.u.). More than 500 nuclei were analyzed from nine animals (n = 9) imaged in vivo. Unpaired and two tailed student t test results in p < 0.0001 (****). j High magnification example of a neuron co-expressing FluoReSyn (green) and hαSyn-mCherry (red), resulting in nuclear colocalization (yellow) of both proteins. Scale bar represents 10 µm. Source data is available as a Source data file.