Fig. 7. Detection of transmittable hαSyn from human CSF samples.

a Illustration of the experimental setup. Reporter-cells or wild-type HEK293 cells (control) were seeded onto glass bottom 96-well plates. FluoReSyn expression was induced by doxycycline for 24 h before they were exposed to human CSF or culture medium (control) for another 24 h. Finally, cells were fixed and ~20 images of random and not overlapping locations were acquired automatically per well. The CSF from each patient was used at least in three different wells. b Quantification of cells with positive FluoReSyn signal. In total, results represent data from 126 wells of Reporter-cells receiving CSF samples from 42 individuals in technical triplicates, 102 negative control wells with Reporter-cells receiving culture medium, 15 wells of HEK293 cells receiving CSF samples from five individuals in technical triplicates and 36 wells of HEK293 cells receiving culture medium. One-way ANOVA with multiple comparison Tukey test ***p < 0.001. Further correlations between the Reporter-cell activity and the clinical information of each patient are displayed in Supplementary Fig. 6 and Supplementary Table 1. Error bars represent the SEM, each data point represents the average from three replicas (wells) of an individual patient’s CSF. Source data is available as a Source data file.