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. 2020 Jun 1;10:8899. doi: 10.1038/s41598-020-65691-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Viability and DNA content of (a,c) NP and (b,d) AF cells at various glucose concentrations under 21% and 5% O₂ on day 6 (n = 9). For viability of NP cells under both 21% and 5% O₂, 5 mM, 3.75 mM, 2.5 mM glucose > 1.25 mM > 0.5 mM (p < 0.05). For viability of AF cells under both 21% and 5% O₂, 5 mM, 3.75 mM, 2.5 mM glucose > 1.25 mM, 0.5 mM glucose (p < 0.05). No statistical significances were found in DNA content among the different treatment groups for both NP and AF cells.