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. 2020 May 26;11:720. doi: 10.3389/fpls.2020.00720

FIGURE 2.

FIGURE 2

Molecular characterization of CaAIMK1. (A) The expression patterns of CaAIMK1 were examined in the leaves of pepper plants after treatment with 100 μM abscisic acid (ABA), drought, 200 mM NaCl, and 100 μM H2O2. The pepper Actin1 gene was used as an internal control. Data represent the mean ± standard error of three independent biological replicates. Asterisks indicate significant differences between 0 h and other times (Student’s t-test; P < 0.05). (B) Subcellular localization of the CaAIMK1-GFP fusion protein in Nicotiana benthamiana epidermal cells. The 35S:CaAIMK1-GFP construct was expressed using agroinfiltration of N. benthamiana leaves and observed under a confocal laser−scanning microscope. White bar = 50 μm. (C) Auto kinase activity of GST-CaAIMK1 and GST-CaAIMK1K32N using [γ-32P]-ATP. GST-OST1 was used as a positive control. The arrows indicates GST-CaAIMK1, GST-CaAIMK1K32N, and GST-OST proteins.