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. 2020 May 26;11:511. doi: 10.3389/fgene.2020.00511

FIGURE 3.

FIGURE 3

The invasive and metastatic properties of KRAS mutated CRC cell lines transfected with MICA *012:01 or MICA *008 allele. The invasive capability of MICA allele overexpressing CRC cell clines was evaluated by transwell assay in (A) SW480 cells, DLD cells, and HCT116 cells. HMF, high magnification field (400 fold) with corresponding histograms (B–D). ns, no significance; *p < 0.05; **p < 0.01. (E) Relative amounts of total MMP-9 and E-cadherin protein levels were analyzed by Western blotting experiments. Representative blots are shown. (F–K) Corresponding histograms of the results are shown. ns, no significance; *p < 0.05; **p < 0.01. (L) The disease-free survival curve of MICA *012:01 and non-MICA *012:01 alleles in CRC patients carrying KRAS codon 12 mutation. Control, untransfected cells; Empty vector, cells transfected with pEGFP-C3 plasmid vector; MICA *008, cells transfected with pEGFP-MICA *008 recombination vector; MICA *012:01, cells transfected with pEGFP-MICA *012:01 recombination vector.