TABLE 1.
Oligonucleotide primers used for RT-qPCR, indicating the location of each primer in the corresponding Ensembl sequences of the Syrian hamster(a).
Gen target | ID transcript ensembl Mesocricetus auratusa | Primer forward | Primer reverse | Size of products | Eb |
Egr1 | ENSMAUG00000007358 | CAGC(A/G)GCGC(T/C)TTCAATCCTC | GTGGTCAGGTGCTCGTAGGG | 60 | 2.04 |
Egr2 | ENSMAUG00000021143 | AGGCCCTTGGATCTCCCATA | CAGCTGGCACCAGGGTACTG | 162 | 2.00 |
Egr3 | ENSMAUG00000000747 | CCACAAGCCCTTCCAGTGTC | GTGCGGATGTGAGTGGTGAG | 75 | 1.98 |
Ttr | ENSMAUG00000011770 | GCCTCGCTGGACTG(G/A)(C/T)ATTT(A/G) | TCGGACAGCATCCAGGACTT | 85 | 2.00 |
Rxfp2 | ENSMAUG00000017558 | AAGCTGTGCCAAAGGTTTCTA | TTGCTGAAAACTTTGACTGGAA | 88 | 1.99 |
C6 | ENSMAUG00000007780 | CTGTGTCCTTGGAGACTACGG | GTCACCAGAGGTTCTGTGCAT | 126 | 1.98 |
Atp2a3 | ENSMAUG00000005976 | TGTGTGGCTGTATGGGTCAT | GCCACGGCAATCTTGAAGTA | 92 | 1.98 |
Rab29 | ENSMAUG00000017388 | TTGCTCTGAAGGTTCTCCAGT | GGCTGTTGCTGAAAGTAGTGG | 168 | 2.06 |
Gm12695 | ENSMAUG00000020018 | GGTCTCCCCAGAAGAAAGTCT | AGGCTGGAGTTCAATGGGTA | 162 | 1.94 |
Grin2c | ENSMAUG00000019504 | GTTCAGCCGTTGGCCTCTAT | ACCCAGATCACACCAGACCT | 90 | 1.90 |
Renbp | ENSMAUG00000019504 | CGAGCACAAGTCATTGACAAA | ATCATGGCTTCACTGTGTGG | 158 | 2.09 |
Slc6a4 | ENSMAUG00000012677 | GCGGTACTGGATGAGTTTCC | TCTATGAGTGCCACCGTGAG | 176 | 1.98 |
Mmp3 | ENSMAUG00000011335 | CCGTGATACCCACCAAATCT | GGGCCAAAATGAAGAGATCA | 92 | 1.93 |
Atf3 | ENSMAUG00000011335 | GGCAACTGGGGAGTCCTTAT | GAGACGAAGGATGCTCTTGC | 120 | 1.98 |
Gadd45g | ENSMAUG00000011335 | TTGCTGTTCTTGGATCGTACA | GACTTTGGCGGACTCGTAGA | 168 | 1.89 |
Slc13a4 | ENSMAUG00000018591 | AGGGGATAGAGCCCATCATC | GCTGACAAACTCCGTGACAA | 165 | 2.07 |
Npas4 | ENSMAUG00000016391 | GGCTACATTCCTTTCCGATG | CTACAAAGTCACCGCAGCAC | 149 | 2.01 |
Wdr38 | ENSMAUG00000005054 | AGCTTCAGCCCTGACTCAAA | GAGGCTGAGTAGCACAAGCA | 65 | 2.07 |
Ogn | ENSMAUG00000011988 | ACCATTGCCAAAGGAATCAG | GTTCTTCTAACAGAGACAGTTTTGAA | 170 | 2.08 |
Sucnr1 | ENSMAUG00000019748 | CAGTCTGTGCCTGACTTTGC | ACAGAGAAGATCGCCACCAC | 164 | 1.95 |
Fos | ENSMAUG00000019419 | CAGCTCGCACCAGTGTCTAC | ACTTCCGGAAAACATCATGG | 76 | 2.00 |
Kcns1 | ENSMAUG00000000788 | CGCTTGTGCGATGATTATGA | ACTCGCCGCTCCAGATAG | 100 | 2.02 |
Junb | ENSMAUG00000004233 | GCAGCTACTTTTCGGGTCAG | TTCATCTTGTGCAGGTCGTC | 200 | 1.97 |
Kcnj13 | ENSMAUG00000010435 | TCAAAGATACCGGAGGATGG | CAAAGACAAGCCAGTGGACA | 178 | 2.07 |
Cd163 | ENSMAUG00000000759 | AGAAGAGAAGCGGAGGGTTC | ACCAGGACAAACTCCAGACG | 163 | 2.03 |
Fosb | ENSMAUG00000000999 | AGAAGAGAAGCGGAGGGTTC | ACCAGGACAAACTCCAGACG | 182 | 2.09 |
Slc28a1 | ENSMAUG00000015388 | TTAATTGCTGCCTCCGTAATG | GAACTTGGACTCCTCCACCTC | 84 | 1.94 |
Actb | ENSMAUG00000008763 | AGCCATGTACGTAGCCATCC | ACCCTCATAGATGGGCACAG | 105 | 2.03 |
qPCR primer efficiency (Eb) was calculated according to the following equation: E = 10(–1/slope).