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. 2020 Mar 19;28(6):1479–1493. doi: 10.1016/j.ymthe.2020.03.010

Figure 2.

Figure 2

Downregulation of LINC01234 Inhibits NSCLC Cell Growth and Induces Apoptosis In Vitro and In Vivo

(A) qRT-PCR analysis of LINC01234 expression in NSCLC cells overexpressing or depleted of LINC01234. (B and C) Growth curves show the proliferation ability of LINC01234-depleted (B) or -overexpressing (C) A549 and SPC-A1 cells. (D) Colony formation assays were used to evaluate the colony formation capacity of LINC01234-depleted A549 and SPC-A1 cells. (E and F) Fluorescence-activated cell sorting (FACS) analysis of the effect of LINC01234 downregulation on cell-cycle progression (E) and apoptosis (F). (G) Western blot analysis of apoptosis- and cell-cycle-related proteins in A549 and SPC-A1 transfected with control or LINC01234-specific siRNA. (H) Representative images of mice-bearing tumors derived from cells expressing empty vector or sh-LINC01234 and tumor volume versus time-growth curves. (I) Tumor weights from two groups are represented. (J) Representative images of tumor samples derived from cells expressing empty vector or sh-LINC01234 subjected to H&E staining, Ki-67 immunostaining, and TUNEL staining. ∗p < 0.05, ∗∗p < 0.01.