Figure 3.

LINC01234 Interacts with RNA-Binding Proteins HNRNPA2B1 and Forms a Complex with DGCR8 in NSCLC Cells

(A) FISH analysis of the subcellular localization of LINC01234 in A549 and SPC-A1 cells. (B) Representative silver-staining image shows the LINC01234 RNA- and anti-sense RNA-precipitated proteins by RNA pull-down assay. (C) GO and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of LINC01234-interacted proteins enriched the cellular process and pathways. (D) The anti-HNRNPA2B1 western blot image shows HNRNPA2B1 binding to desthiobiotinylated LINC01234 in A549 cells. (E) Schematic representation of the full-length LINC01234 and its truncations. The anti-HNRNPA2B1 western blot image shows HNRNPA2B1 binding to desthiobiotinylated full-length and different truncations of LINC01234 in A549 cells. (F) RIP assays showing LINC01234 and LNMAT2 coimmunoprecipitation (coIP) with HNRNPA2B1 from A549 and SPC-A1 cells. (G) RIP assays showing LINC01234 coimmunoprecipitation with HNRNPA2B1 in LINC01234 or control siRNA-transfected A549 and SPC-A1 cells. (H) CoIP assays show the interaction between HNRNPA2B1 and DGCR8 in A549 and SPC-A1 cells. ∗∗p < 0.01.