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. 2020 Mar 19;28(6):1479–1493. doi: 10.1016/j.ymthe.2020.03.010

Figure 8.

Figure 8

c-Myc Promotes LINC01234 Transcription in NSCLC Cells

(A) Prediction of c-Myc-binding sites in the promoter region of LINC01234 by JASPAR. (B) Gene set enrichment analysis (GSEA) analysis shows the enrichment of LINC01234-regulated genes in the c-Myc target list. (C) Correlation between c-Myc and LINC01234 expression in the GEO: GSE18842 dataset. (D) qRT-PCR analysis of LINC01234 expression in A549, SPC-A1, and HEK293T cells transfected with a c-Myc-overexpression vector or siRNA. (E) ChIP-qPCR analysis of c-Myc occupancy of the LINC01234 promoter in A549 and SPC-A1 cells. H19 was used as a positive control and IgG as a negative control. (F) Dual-Luciferase reporter assays of c-Myc binding to the LINC01234 promoter region. (G) Proposed model illustrated the modulatory role of c-Myc-activated LINC01234 acting as an oncogenic lncRNA that interacts with HNRNPA2B1 and regulates miR-106b biogenesis. ∗p < 0.05, ∗∗p < 0.01.