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. 2020 Apr 14;28(6):1506–1517. doi: 10.1016/j.ymthe.2020.04.006

Figure 5.

Figure 5

circRNA Offers Superior Protection against Endogenous Nuclease Degradation

RNA stability assay of sponge constructs in HEK293T cells following actinomycin D treatment. 18S and MYC act as endogenous control transcripts while mCherry is a plasmid driven control transcript. RNA levels at each time point were measured by qPCR, to enable comparison of RNA stability between (A) plasmid-driven circmiRs versus linear sponges, and (B) in vitro synthesized circmiRs versus linear sponges. ∗∗p < 0.01 and ∗∗∗∗p < 0.0001 (circmiR versus linear sponge). One-way ANOVA with Benjamini-Hochberg adjustment.