Figure 7.

AAV-Mediated GFP Expression in Macaques that Received Extrafoveal Subretinal Injections

Retinal cross-sections from macaque eyes that received extrafoveal subretinal injections (SRI) of AAV44.9-hGRK1-GFP (A and B) or AAV44.9(E531D)-hGRK1-GFP (C and D) were immunostained with an antibody raised against cone arrestin (red) and counterstained with DAPI (blue). GFP expression (green) was absent from parafoveal cones (~550 mM nasal or temporal from the foveal pit) in eyes injected with AAV44.9. In contrast, parafoveal cones efficiently expressed AAV44.9(E531D)-mediated GFP. ×20 original magnification (A and C) and ×40 original magnification (C and D) images are shown. Scale bars in (A) and (C), 40 μm, and in (B) and (D), 20 μm. RPE, retinal pigment epithelium, IS/OS, inner segments/outer segments; ONL, outer nuclear layer; INL, inner nuclear layer.