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. 2020 Apr 11;28(6):1494–1505. doi: 10.1016/j.ymthe.2020.04.001

Figure 2.

Figure 2

miR-1293 Directly Targets the 3′ UTR of BRD4

(A) Western blot analysis of BRD4 in HCT116+/+, HCT116−/−, and HOC313 cells 48 h after transfection with 10 nmol/L miR-NC or the indicated 7 miRNAs. (B) Knocking down BRD4 suppressed in vitro cell proliferation in HCT116+/+, HCT116−/−, and HOC313 cells. Western blot analysis (right) and cell growth assay (left) in each cell line after transfection with 20 nmol/L negative control siRNA (si-NC) or siRNA targeting BRD4 (si-BRD4). ∗p < 0.05. (C–E) Luciferase reporter assay. HOC313 cells were cotransfected with pmirGLO dual-luciferase vectors containing wild-type (WT) BRD4-3′ UTR or mutant variants of BRD4-3′ UTR and miR-NC, miR-1293 (C), miR-876-3p (D), or miR-6751-5p (E). Top, putative binding sequences of each miRNA within the 3′ UTR of BRD4 and mutant sequences are indicated. Bottom, the results of the luciferase assay; ∗p < 0.05.