Table 1.
Details of product PCR size for CYP2C19∗2, CYP2C19∗3, and CYP2C19∗17 PCR and RFLP characteristics. CYP2C19∗17 analyses consisted of a nested PCR (PCR 1 and PCR 2).
| Allele | PCR product size (bp) | Enzyme | Wild-type (size bp) | Heterozygous (size bp) | Homozygous mutant (size bp) | Forward primer (5’ – 3′) | Reverse primer (5’ – 3′) |
|---|---|---|---|---|---|---|---|
| 2C19∗2 | 223 | SmaI | 113–110 | 223-113-110 | 223 | CAACCAGAGCTTGGCATATTG | TAAAGTCCCGAGGGTTGTTG |
| 2C19∗3 | 271 | BamHI | 175–96 | 271-175-96 | 271 | AAATTGTTTCCAATCATTTAGCT | ACTTCAGGGCTTGGTCAATA |
| 2C19∗17 (PCR 1) | 470 | --- | --- | --- | --- | GCCCTTAGCACCAAATTCTC | ATTTAACCCCCTAAAAAAACACG |
| Nested (PCR 2) | 143 | NsiI | 166–27 | 143-116-27 | 143 | AAATTTGTGTCTTCTGTTCTCAATG | AGACCCTGGGAGAACAGGAC |