(a) Quantification of crystal violet staining to indirectly measure the amount of surface attached biomass (OD600) of LAB isolates C3, C4, C6, C8, and F6. Error bar indicates the standard deviation (n = 3). Bars marked with a, b, and c had the three highest activity levels. Based on ANOVA, the different letters represent a significant difference of p < 0.05.(b) Bright-field microscopy of flow cell inoculated with different LAB strains and the subsequent biofilm attachment and morphology after one day of growth at ambient temperature with a constant flow rate of 0.2 ml/ minute. The scale bar represents 0.1 mm while the pictures were captured at 300 × .