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. 2020 May 26;11:1068. doi: 10.3389/fmicb.2020.01068

FIGURE 2.

FIGURE 2

Virulence attenuation on Pseudomonas aeruginosa by PLFE1. (A) Anti-virulence effects of PLFE1 in human A549 lung cells infection model. The presence of PLFE1 (100 μg mL–1) significantly protected A549 lung cells from lysis after 20 h infection. Data are presented as the mean ± SEM values of four independent experiments performed in duplicate. ** P = 0.001 to 0.01 (two-tailed t test) versus untreated cells. (B) Pseudomonas aeruginosa H103 virulence attenuation in a C. elegans infection model by PLFE1. Sixty L4-stage nematodes per experimental group were placed on lawns of E. coli OP50 (black) or H103 strain in the absence (red) or presence of PLFE1 at 100 μg mL–1 (green). Alive nematodes were scored at 4-, 6-, 8-, 20- and 24-h after the start of the assay. ****, P < 0.0001 (log-rank [Mantel-Cox] test) versus untreated cells.