Figure 4.

Immunoprecipitation of OAS2 in cells. (A) Immunoprecipitation of OAS2 using anti-His antibody is shown. HEK293T cells were transfected with empty vector, FLAG-OAS2, HIS-OAS2, and in combination with both. Cells were lysed and protein immunoprecipitated using anti-His antibodies and IgG negative control. Immunoprecipitated (IP) protein was loaded on the 10% SDS-PAGE gel, and equal amounts of protein were loaded before (pre-IP) and after (post-IP). The Western blot was performed using anti-FLAG antibodies. (B) Immunoprecipitation of OAS2 using FLAG antibodies. The same immunoprecipitation procedure was followed as in (A), except anti-FLAG antibodies were used for immunoprecipitation and the Western blot was performed using anti-His antibodies.