Table 2.
Experimental and Predicted Hydrodynamic Parameters for Recombinant OAS1
| Peak-1 | Peak-2 | |
|---|---|---|
| Guinier Data | ||
| rG (Å) | 34.10 ± 0.10 | 24.88 ± 0.02 |
| I0 | 0.0058 ± 1.7 × 105 | 0.014 ± 4.2 × 105 |
| q × rG range | 0.29–1.30 | 0.31–1.28 |
| Data points | 6–133 | 28–195 |
| Real-space data | ||
| rG (Å) | 35.66 ± 0.10 | 24.62 ± 0.04 |
| Dmax (Å) | 110 | 70 |
| I0 | 0.0057 ± 1.9 × 105 | 0.013 ± 2.6 × 105 |
| q range (Å−1) | 0.009–0.346 | 0.017–0.30 |
| Sequence Mw (kDa) | 41.74 | 41.74 |
| Mw calculated (kDa) SAXS MoW | 78, dimer | 39, monomer |
| s20, w (S) | 5.1 ± 0.3 | 3.2 ± 0.2 |
| rH (nm) | N/A | 3.83 ± 0.05 |
| χ2 DAMMIN | ∼0.70 | ∼1.40 |
| NSD DAMMIN | 1.0 ± 0.05 | 0.71 ± 0.08 |