. 2020 May 3;13(4):1273–1283. doi: 10.1111/1751-7915.13574

Table 1.

Parameters determined in the cultures with Pseudomonas putida EM42 resting cells and in the cultures with P. putida EM42 or P. putida EM42 pSEVA2213_bglC grown on d‐glucose or d‐cellobiose, respectively, and transforming d‐xylose to d‐xylonate.

P. putida strain and culture conditions	μ (h⁻¹) ^e	Xylonate yield (g g⁻¹ xylose)	Xylonate productivity (mg l⁻¹ h⁻¹)	CDW (g)	pH
EM42 resting cells opt ^a	n.a.	0.56 ± 0.06/0.85 ± 0.06	217 ± 15/164 ± 11	0.15 ± 0.01/0.16 ± 0.01	6.30 ± 0.01/5.99 ± 0.08
EM42 glucose	0.58 ± 0.02	0.18 ± 0.02/0.17 ± 0.03	69 ± 6/32 ± 6	1.73 ± 0.12/1.60 ± 0.06	6.26 ± 0.01/5.81 ± 0.03
bglC⁺ EM42 cellobiose	0.27 ± 0.03	0.30 ± 0.06/0.48 ± 0.09	114 ± 18/93 ± 13	1.46 ± 0.13/1.88 ± 0.05	6.16 ± 0.06/5.19 ± 0.03
bglC⁺ EM42 cellobiose optA ^b	0.28 ± 0.05	0.34 ± 0.05/0.54 ± 0.10	138 ± 21/107 ± 17	2.06 ± 0.07/1.85 ± 0.03	6.23 ± 0.07/5.88 ± 0.05
bglC⁺ EM42 cellobiose optB ^c	0.30 ± 0.02	0.35 ± 0.02/0.50 ± 0.01	144 ± 8/102 ± 3	2.41 ± 0.11/2.18 ± 0.07	6.27 ± 0.08/6.00 ± 0.04
bglC⁺ EM42 cellobiose PHA ^d	0.24 ± 0.01	0.41 ± 0.09/0.52 ± 0.08	156 ± 32/99 ± 13	0.86 ± 0.02/1.24 ± 0.14	6.24 ± 0.02/5.90 ± 0.01

Values represent the mean ± standard deviation of three biological replicates. Parameters (except for μ) were determined after 24 h/48 h of the culture. CDW, cell dry weight; n.a., not applicable.

^{^a}

Resting cells, pre‐cultured in LB medium, were incubated in flasks with M9 minimal medium buffered with 100 mM sodium phosphate buffer and shaken at 300 r.p.m.

^{^b}

Cultures, inoculated from pre‐cultures grown in LB medium, were carried out in flasks with M9 minimal medium buffered with 100 mM sodium phosphate buffer and shaken at 300 r.p.m.

^{^c}

Cultures, inoculated from pre‐cultures grown in M9 medium with d‐cellobiose, were carried out in flasks with M9 minimal medium buffered with 100 mM sodium phosphate buffer and shaken at 300 r.p.m.

^{^d}

Cultures were carried out in flasks with M9 minimal medium with reduced content of nitrogen, buffered with 100 mM sodium phosphate buffer and shaken at 300 r.p.m.

^{^e}

The specific growth rate (μ) was determined during exponential growth.