Fig. 5.

Deletion of pfiT activates Pf4 production.

A. Pf4 phage titres were determined on ΔPf4 lawns using pellicle supernatants from strains PAO1 and ΔpfiT. Strains were cultured in 6‐well plates under static conditions. Three independent cultures of each strain were used, and error bars indicate standard deviation. Plaque formation by the phage lysates at 6 h is also shown. Phage lysates were serially diluted, and 10 μl samples were spotted on ΔPf4 lawns.

B. Relative fold changes of mRNA of pfiA, pf4r, xisF4 and PA0727 in strain ΔpfiT versus wild‐type strain PAO1. RNA was extracted from the static pellicle culture at 6 h.