Table 2.
Identification of two representative bands of MV proteins
| Rank a | Name | Locus Tag b | MASCOT score a | Theoretical pI/MW c | Subcellular localization d | Lipoprotein signal peptide e | Signal peptide f |
|---|---|---|---|---|---|---|---|
| Band ID #1 at 15 kDa | |||||||
| 1 | Type‐1 fimbrial protein subunit A, FimA | ECOLIN_05555 | 480 | 5.12/15873.56 | Extracellular | No | Yes/24 |
| 2 | Type‐1 fimbrial protein protein G precursor, FocG | ECOLIN_05580 | 244 | 8.66/15187.76 | Non‐cytoplasm | No | Yes/22 |
| 3 | Outer membrane lipoprotein, SlyB | ECOLIN_08845 | 202 | 8.93/15627.66 | Outer membrane | Yes/17 | No |
| Band ID #2 at 100 kDa | |||||||
| 1 | Acetaldehyde dehydrogenase | ECOLIN_07285 | 1134 | 6.32/96058.13 | Cytoplasm | No | No |
a
The list of proteins is ranked by MASCOT score. All candidates with MASCOT score greater than 200 and more than two peptide hits.
b
Locus tags in the whole genome database of Escherichia coli Nissle 1917 (NCBI DB Accession: CP007799.1).
c
Theoretical pI/molecular weight predicted by ExPaSy.
d
Cellular localization predicted by PSORTb 3.02 program.
e
Presence/length of lipoprotein signal peptides predicted by LipoP 1.0 program.
f
Presence/length of signal peptides predicted by SignalP 4.1 program. Total peptide numbers are also shown in parentheses.