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. 2020 Feb 16;13(4):997–1011. doi: 10.1111/1751-7915.13544

Figure 1.

Figure 1

Photosynthetic co‐culture of engineered Synechococcus elongatus and Pseudomonas putida designed for simultaneous biodegradation and bioproduction. Selongatus CscB, encapsulated within alginate beads, fix carbon dioxide via the Calvin‐Benson‐Bassham cycle driven by photosynthetically active radiation (PAR). The fixed carbon is converted into sucrose and exported through a heterologous sucrose permease (CscB) into the culture medium. The exported sucrose is consumed by P. putida EM·DNT·S for growth, supporting biotransformation of the toxic pollutant 2,4‐dinitrotoluene (2,4‐DNT), and concurrent production of polyhydroxyalkanoate (PHA).