Main metabolic pathway for glycerol conversion by Thermoanaerobacter brockii subsp. finnii (DSM 3389T) and Thermoanaerobacter wiegelii (DSM 10319T). Grey lines: absent in Thermoanaerobacter. Broken lines mean multiple steps. Abbreviations: GLY, glycerol; 3HPA, 3‐hydroxypropionaldehyde; 1,3‐PDO, 1,3‐propanediol; DHA, dihydroxyacetone; DHAP, dihydroxyacetone phosphate; G3P, glycerol‐3‐phosphate; GA3P, glyceraldehyde‐3‐phosphate; PEP, phosphoenolpyruvate; PYR, pyruvate; LAC, lactate; AcCOA, acetyl‐coenzyme A; FOR, formate; ACE, acetate; EtOH, ethanol; Fdox, oxidized ferredoxin; Fdred, reduced ferredoxin. 1, glycerol dehydratase; 2, 1,3‐PDO dehydrogenase; 3, glycerol dehydrogenase; 4, dihydroxyacetone kinase; 5, glycerol kinase; 6, glycerol‐3‐phosphate dehydrogenase; 7, triosephosphate isomerase; 8, glyceraldehyde‐3‐phosphate dehydrogenase, phosphoglycerate kinase, phosphoglycerate mutase and enolase; 9, pyruvate kinase; 10, L‐lactate dehydrogenase; 11, pyruvate:ferredoxin oxidoreductase; 12, pyruvate formate lyase; 13, formate hydrogen lyase; 14, phosphate acetyltransferase and acetate kinase; 15, acetaldehyde dehydrogenase; 16, hydrogenase; and 17, ferredoxin‐NADP(+) reductase. Additional information of EC number and genome location of the enzymes of this metabolic pathway can be found at Table S2.