Fig. 2.

Representative plots illustrating the gating strategy. A) Representative flow cytometry plots of CD3 expression on CD45 + cells. B) Percentage of circulating CD45⁺ CD3⁺ cells measured by flow cytometry 38 days after intraplantar saline or CFA injection (n = 5 mice/group). C) Representative flow cytometry plots of CD4 and CD8 expression on CD3 + cells isolated from WT 38 days after CFA injection. D) Percentage of circulating CD3⁺CD4⁺ and CD3⁺CD8⁺ cells measured by flow cytometry 38 days after intraplantar saline or CFA injection (n = 5 mice/group). Significant statistical difference was indicated by * = p < 0.05, ** = p < 0.01 and *** = p < 0.001. Data are presented as mean ± standard error of the mean.