Table 2.
Experimental protocol.
Group A: Saline | Mice were intraperitoneally (i.p.) injected with saline once per week over a period of 12 weeks. Saline is the vehicle used to dissolve DMH. |
Group B: Extract | Mice received M. chamomilla aqueous extract only at 150 mg/Kg/ b.w. by gavage (P.O.) 5 days/week for 12 weeks. |
Group C: DMH 12 weeks | Mice received DMH dissolved in saline (20 mg/Kg b.w., i.p.) once per week over 12 weeks to induce colorectal cancer (CRC). |
Group D: Pre-treatment | Mice were pre-treated with M. chamomilla extract (150 mg/Kg/ b.w.) starting 1 week before DMH injections and continued till 1 week after the final DMH exposure. |
Group E: DMH 24 weeks | Mice received DMH for 12 weeks as in group C and left without any treatment for an additional 12 weeks. |
Group F: Post-treatment | Mice received M. chamomilla extract (150 mg/Kg/ b.w.) starting 1 week after the twelfth DMH injection and continued over additional 12 weeks. |