Table 3.
Sequences of forward and reverse primers used to amplify the selected genes.
| Gene | Primer Sequence | Product size (bp) |
|---|---|---|
| GAPDH |
F: 5′-TGGTGCTCAGTGTAGCCCAG-3′ R: 5′-GGACCTGACCTGCCGTCTAG-3′ |
111 |
| Wnt5a |
F: 5′-CTGGCAGGACTTTCTCAAGG-3′ R: 5′-CTCTAGCGTCCACGAACTCC-3′ |
395 |
| GSK3β |
F: 5′-TCCATTCCTTTGGAATCTGC-3′ R: 5′-CAATTCAGCCAACACACAGC-3 |
236 |
| APC |
F: 5′-TGGAAGTGTGAAAGCATTGATGGAATGTGC-3′ R: 5′-CCACATGCATTACTGACTATTGTCAAG-3′ |
348 |
| β-Catenin |
F: 5′-GCTGACCTGATGGAGTTGGA-3′ R: 5′-GCTACTTGCTCTTGCGTGAA-3′ |
227 |
| Lef1 |
F: 5′-TGAGTGCACGCTAAAGGAGA-3′ R: 5′-ATAATTGTCTCGCGCTGACC-3′ |
160 |
| Tcf4 |
F: 5′-CAAAGAAAGTCCGAAAAGTTCCT-3′ R: 5′-GGCGAGTCCCTGTTGTAGTC-3′ |
88 |
| C-Myc |
F: 5′-TAGTGCTGCATGAGGAGACA-3′ R: 5′-GGTTTGCCTCTTCTCCACAG-3′ |
104 |
| Cyclin D1 |
F: 5′-GGCACCTGGATTGTTCTGTT-3′ R: 5′-CAGCTTGCTAGGGAACTTGG-3′ |
232 |
F: Forward set; R: Reverse set; bp = base pair.