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. 2020 May 26:1–5. doi: 10.1080/1061186X.2020.1769637

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Figure 3. — Diagnostic applications of CRISPR-Cas13 based SHERLOCK technique for COVID-19. The protocol includes three steps: Step (I): isothermal amplification of the extracted nucleic acid sample using a commercially available recombinase polymerase amplification (RPA) kit (25 min incubation); Step (II): detection of pre-amplified viral RNA sequence using Cas13 (30 min incubation); Step (III): visual read out of the detection result by eye using a commercially-available paper dipstick (2 min incubation).