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. 2020 Apr 23;39(11):e101573. doi: 10.15252/embj.2019101573

Figure EV4. Accumulation of PAR in response to H2O2 in PC3 and HME cells.

Figure EV4

  1. PC3 DKO cells were treated with H2O2 for 10, 15, or 20 min, and analyzed by the Western method for PAR accumulation. Ponceau staining of the membrane is shown as a loading control.
  2. HME DKO cells were treated with H2O2 for 0, 12, or 17 min, and analyzed by the Western method for PAR accumulation and PARP1 migration. High exposure for PARP1 shows slowly migrating PARP1‐PAR molecules. Ponceau staining of the membrane is shown as a loading control.
  3. Western analysis of lysates from HME cells, pre‐exposed to IFNβ for 0–3 days, and treated with H2O2 for 15 min. Top: Induction of OAS1‐3 after IFNβ treatment. Bottom: Accumulation of PAR in cells pre‐treated with IFNβ for different time. GAPDH staining is shown as a loading control.