Figure EV3. Analysis of Kinetochore tension and segregation.
Immunofluorescence images from mitotic (mitosis, top two panels) and ana/telophase (Exit, bottom two panels) Ctr‐ and DIA‐treated B1dd/B2ko cells stained with anti‐CenpA (green) and anti‐CenpB (red) antibodies. For DIA‐treated cells, we determined the exit state by the onset of a cytokinesis furrow, since sister‐chromatid segregation did not occur. Areas marked by squares are enlarged twofold at the right of each panel. The scale bar represents 5 μm.