Figure EV1. VSV infection does not influence protein level, translocation of SIRT5, but attenuates SIRT5 binding to MAVS; VSV infection diminishes K63‐linked polyubiquitination of MAVS .

1. Western blot analysis of SIRT5 in H1299 cells infected with VSV at different time points (0, 2, 4, 6, 8, 12 h).
2. Western blot analysis of SIRT5 in mitochondria, cytoplasm, and nuclei of H1299 cell infected with VSV at different time points (0, 4, 8 h).
3. Western blot analysis for SIRT5 binding to MAVS in H1299 cells infected with VSV at different time points (0, 2, 4, 8 h).
4. VSV infection caused a reduction of intracellular succinate level.
5. Overexpression of SIRT5 attenuated polyubiquitination of MAVS in HEK293T cells.
6. Overexpression of SIRT5 attenuated K63‐linked polyubiquitination of MAVS in HEK293T cells.
7. Knockout of SIRT5 in H1299 cells enhanced K63‐linked polyubiquitination of endogenous MAVS in response to VSV infection.

Source data are available online for this figure.