Figure EV2. SIRT5 negatively regulates cellular antiviral response.

• A
  qRT–PCR analysis of IFNβ mRNA in HEK293T cell transfected with Myc empty vector or Myc‐SIRT5 for 24 h, followed by infected with SeV in an increasing titration (+, ++) for 8 h. −, without infection.
• B–D
  qRT–PCR analysis of IFNβ (B), CXCL10 (C), and IFIT1 (D) mRNA in SIRT5‐deficient (SIRT5 ^−/−) or WT (SIRT5 ^+/+) H1299 cells infected with SeV in an increasing titration (+, ++) for 8 h. −, without infection.
• E–G
  qPCR analysis of IFNβ (E), CXCL10 (F), and IFIT1 (G) mRNA in SIRT5‐deficient (SIRT5 ^−/−) or WT (SIRT5 ^+/+) H1299 cells infected with SeV for different time points (0, 4, 8 h).
• H, I
  qPCR analysis of IFNβ (H) and CXCL10 (I) mRNA in SIRT5‐deficient H1299 cells (SIRT5 ^−/−) transfected with the control plasmid (Myc empty), the plasmid expressing Myc‐SIRT5 (Myc‐SIRT5), or the plasmid expressing the enzyme‐deficient mutant H158Y (Myc‐SIRT5‐H158Y) for 24 h, followed by infection with (+) or without (−) VSV for 8 h.
• J
  Western blot analysis for Fig 4M.
• K
  Western blot analysis for Fig 4N.
• L
  Fluorescence microscopy images of VSV‐GFP virus replication in SIRT5‐deficient H1299 cells (SIRT5 ^−/−) transfected with the control plasmid (Myc empty), the plasmid expressing Myc‐SIRT5 (Myc‐SIRT5), or the plasmid expressing the enzyme‐deficient mutant H158Y (Myc‐SIRT5‐H158Y) for 24 h, followed by infection with VSV‐GFP (MOI = 0.1) for 12 h.