Figure EV3. SIRT5 deficiency enhances cellular antiviral response in HCT116 cells.

• A–D
  qPCR analysis of IFNβ (A), ISG15 (B), CXCL10 (C), and IFIT1 (D) mRNA in SIRT5‐deficient (SIRT5 ^−/−) or WT (SIRT5 ^+/+) HCT116 cells infected with or without SeV (Sev or UI) for 8 h. UI, uninfected; the graphs represent the fold induction relative to the untreated control cells. All data are presented as the mean values based on three independent experiments, and error bars indicate s.e.m.
• E
  Fluorescence microscopy images of VSV‐GFP virus replication in SIRT5‐deficient (SIRT5 ^−/−) or WT (SIRT5 ^+/+) HCT116 cells after infected with VSV‐GFP viruses (MOI = 0.1) for 12 h (fluorescence, upper; bright‐field, bottom). The expression of SIRT5 in SIRT5‐deficient (SIRT5 ^−/−) or WT (SIRT5 ^+/+) HCT116 cells was examined by Western blot analysis.