Figure EV5. Sirt5 deficiency potentiates antiviral immune response in spleen.

• A–E
  qPCR analysis of Ifnβ (A), Ifnα1 (B), Isg15 (C), Cxcl10 (D), and Cxcl11 (E) mRNA in the spleens of WT (Sirt5 ^+/+) or Sirt5‐deficient (Sirt5 ^−/−) mice injected intraperitoneally with VSV (1 × 10⁷ plaque‐forming units (PFU) per mouse) or PBS control for 24 h.
• F
  qPCR of VSV mRNA in the spleens of WT (Sirt5 ^+/+) or Sirt5‐deficient (Sirt5 ^−/−) mice injected intraperitoneally with VSV (1 × 10⁷ plaque‐forming units (PFU) per mouse) or PBS control for 24 h.

Data information: PBS, phosphate‐buffered saline. The graphs represent the fold induction relative to the untreated WT mice. All data are presented as the mean values based on three independent experiments, and error bars indicate s.e.m. (unpaired two‐tailed Student's t‐test).