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. 2020 Jun 2;20:9. doi: 10.1186/s12861-020-00216-2

Table 3.

Primer sequences for target genes

Target gene Sequence (5′ – 3′) Product length (bp) Accession no.
Notch1

Fwd: ACAGTAACCCCTGCATCCAC

Rev.: GGTTGGACTCACACTCGTTG

120 NM_008714.3
Notch2

Fwd: GACTGCACAGAAGACGTGGA

Rev.: GCGTAGCCCTTCAGACACTC

116 NM_010928.2
Notch3

Fwd: GTGTCAATGGTGGTGTCTGC

Rev.: GCACACTCATCCACATCCAG

103 NM_008716.2
Notch4

Fwd: GAGGGACACTCCACCTTTCA

Rev.: CTGGTGCCTGACACAGTCAT

93 NM_010929.2
Delta-like1

Fwd: GTTGTCTCCATGGCACCTG

Rev.: TGCACGGCTTATGGTGAGTA

111 NM_007865.3
Delta-like4

Fwd: GGAACCTTCTCACTCAACATCC

Rev.: CTCGTCTGTTCGCCAAATCT

141 NM_019454.3
Jagged1

Fwd: CCAGCCAGTGAAGACCAAGT

Rev.: CAATTCGCTGCAAATGTGTT

127 NM_013822.5
Jagged2

Fwd: AGTGCCATCTGGCTTTGAAT

Rev.: CGCTGCACATGGGTTAGAG

97 NM_010588.2
Hes1

Fwd: GCGAAGGGCAAGAATAAATG

Rev.: TGTCTGCCTTCTCTAGCTTGG

104 NM_008235.2
Hes2

Fwd: CGGATCAACGAGAGCCTAAG

Rev.: GTCTGCCTTCTCCAACTTCG

93 NM_001301805.1; NM_008236.4
Sox2

Fwd: GGTTCTTGCTGGGTTTTGATTCT

Rev.: CCTTCCTTGTTTGTAACGGTCCT

59 NM_011443.4
Klf4

Fwd: GCAGTCACAAGTCCCCTCTC

Rev.: GACCTTCTTCCCCTCTTTGG

93 NM_010637.3
Oct4

Fwd: TGGAGGAAGCCGACAACAAT

Rev.: GCTGATTGGCGATGTGAGTG

177 NM_001252452.1; NM_013633.3
Cdx2

Fwd: CTGGCTCCGCAGAACTTTGT

Rev.: GGTGCGTAGCCATTCCAGTC

170 NM_007673.3
Cdca7

Fwd: ACA TGC TGG TGA GAC AGA GGA A

Rev.: TAT ATG CGG AAG GGT CAT GGA

98 NM_025866.3
Lgr5

Fwd: CCC ATC CAA TTT GTT GGA GTA

Rev.: GTG GCA GTT CCT GTC AAG TG

113 NM_010195.2
Rps29

Fwd: CACGGTCTGATCCGCAAATAC

Rev.: ACTAGCATGATCGGTTCCACTTG

144 NM_009093.2
Hprt1

Fwd: GTCGTGATTAGCGATGATGAACC

Rev.: GCAAGTCTTTCAGTCCTGTCCATAA

128 NM_013556.2