Fig. 2. Cav3.1 and Kv4.3 channels co-associate with FMRP.

a Co-immunoprecipitation (coIP) of FMRP with Cav3.1 or Kv4.3 from whole brain lysates of WT mice. Immunoprecipitation (IP) was conducted using rabbit anti-Cav3.1 and rabbit anti-Kv4.3 antibodies and rabbit IgG (as control), and immunoblotting using a mouse anti-FMRP N-terminus antibody. All coIP experiments were derived from at least three different experiments. b, c tsA-201 cells coexpressing GFP-Cav3.1 and FMRP-mKate as a donor–acceptor pair exhibit FRET upon excitation of GFP at 457 nm (b solid arrows). A representative example of peak fluorescent emissions for both GFP and mKate upon excitation at 457 nm is shown in (c). No dual emissions are detected in cells expressing only GFP-Cav3.1 (open arrows) or in cells expressing both GFP-Cav3.1 and FMRP-mKate when excited at 561 nm (solid arrows) (b). d, e tsA-201 cells coexpressing GFP-Kv4.3 and FMRP-mKate exhibit FRET upon excitation of GFP at 457 nm (solid arrows) that is absent in cells expressing only GFP-Cav3.1 (open arrows) (d). A representative example of peak fluorescent emissions for both GFP and mKate upon excitation at 457 nm for cells is shown in (e). Scale bars in (b, d), 10 μm. All FRET experiments were derived from three different experiments with at least three different dishes of cells. Source data are provided as a Source Data file.