Skip to main content
. 2020 Jun 2;11:2755. doi: 10.1038/s41467-020-16250-4

Fig. 9. FMRP(1–297)-tat can restore levels of protein translation in Fmr1 KO mice.

Fig. 9

Shown are representative Western blots and mean bar-dot plots of the density of bands for the indicated proteins detected in cerebellum or brain samples from P21–30 WT or Fmr1 KO mice 24 h following a single tail vein injection of either vehicle or 1.0 mg/kg HA-FMRP(1–297)-tat. Protein levels were normalized to GAPDH or vinculin. a Fmr1 KO mice exhibit elevated levels of αCaMKII that is significantly reduced 24 h following injection of HA-FMRP(1–297)-tat in samples from cerebellum but not in brain (two-way ANOVA analysis of the interaction between genotype and treatment: cerebellum, F(1,18), interaction = 10.50, p = 0.0045, post hoc Tukey’s test p(KO veh, WT veh) < 0.0001, p(KO veh, KO HA) = 0.0448; brain, F(1,18), interaction = 7.161, p = 0.0154, post hoc Tukey’s test p(KO veh, WT veh) = 0.0176, p(KO veh, KO HA) = 0.113). b Fmr1 KO mice exhibit elevated levels of APP that is significantly reduced by HA-FMRP(1–297)-tat injection in both cerebellum and brain (two-way ANOVA: cerebellum, F(1,18), interaction = 6.176, p = 0.0230, post hoc Tukey’s test p(KO veh, WT veh) = 0.0076, p(KO veh, KO HA) = 0.0438; brain, F(1,18), interaction = 2.272, p = 0.1491, F(1,18), treatment = 8.204, p = 0.0103, F(1,18),genotype = 19.05, p = 0.0004, post hoc Tukey’s test p(KO veh,WT veh) = 0.0031, p(KO veh,KO HA) = 0.0293). c Fmr1 KO mice exhibit reduced levels of PSD95 protein in cerebellum but not in brain (cerebellum, two groups of lysates ran on one blot, two-sample t test, t(6) = −2.636. p = 0.039; brain, all four groups of lysates ran on one blot, two-way ANOVA analysis, F(1,12),interaction = 0.00417, p = 0.950, F(1,12),treatment = 2.820, p = 0.119, F(1,12),genotype = 3.630, p = 0.0810, no significant difference among two-group comparisons). Samples after injection of HA-FMRP(1–297)-tat showed no significant difference between WT and Fmr1 KO cerebellum (two-sample t test, t(6) = 0.174. p = 0.867). Average values are mean ± s.e.m, and sample values identified at the base of bar plots represent the number of animals. *p < 0.05, ***p < 0.001. NS not significant. Source data are provided as a Source Data file.