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. 2020 Jun 2;10:8971. doi: 10.1038/s41598-020-65708-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gain-of-function assay of novel_miR_1 in vivo. Gain-of-function assay was conducted in vivo by injection of novel_miR_1 mimics. At 3d post injection, qRT-PCR was performed to evaluate the expression levels of novel_miR_1, putative target genes and several biomineralization-related genes in mantle tissues. (a–i) Relative expression level of novel_miR_1, Prisilkin-39, ACCBP, PfCHS1, PfMG11, N151, Nacrein, Shematrin-2, KRMP. The RNA-free water group was used as control. The results were analyzed by Student’s T test.