Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 12;219(6):e201906153. doi: 10.1083/jcb.201906153

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Bartle et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — Dsg3 trans binding is critical in cadherin order and dynamics. (A) HaCaT cells were transfected with Dsg3-ΔEA-GFP (Dsg3-wt) or Dsg3-ΔEA-GFP-W2A (Dsg3-W2A) and imaged with fluorescence polarization microscopy. (B) Mean order factor (Dsg3-W2A n = 7; Dsg3-wt n = 19; mean ± SD; **, P ≤ 0.01; Student’s t test). (C and D) FRAP experiments were conducted on HaCaT cells cotransfected with Dsg3-W2A and DP-mCherry and mock treated in normal-Ca²⁺ medium. (C) Representative cell and time points of region of interest (ROIs). (D) Fluorescence recovery over time and mobile fraction of the ROI. (E and F) FRAP experiments were conducted on HaCaT cells cotransfected Dsg3-W2A and DP-mCherry, treated with Gö6976, and switched to low-Ca²⁺ medium. (E) Representative cell and time points of ROI. (F) Fluorescence recovery over time and mobile fraction of the ROI. (G and H) FRAP recovery curves (mean ± SD; G) and mobile fractions (mean ± SD; H) for cells cotransfected with both Dsg3-W2A and DP-mCherry with mock (n = 9) or Gö6976 (n = 11) treatment (ns, not significant, P > 0.05; *, P ≤ 0.05; **, P ≤ 0.01; ANOVA). (I) Schematic of how trans binding changes cadherin mobility in hyperadhesive desmosomes. Scale bars = 5 µm; ROI scale bars =1 µm.