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. 2020 May 12;219(6):e201906153. doi: 10.1083/jcb.201906153

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© 2020 Bartle et al.

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Figure S2. — Representative images of cells expressing Dsg2-mCherry showing that exchange is reduced in hyperadhesion. Representative cells from data presented in Fig 4. (A) HaCaT cell transfected with Dsg2-mCherry, mock treated, and maintained in normal Ca²⁺ with bleach region of interest (ROI) indicated. Scale bar = 5 µm. (B) Zoom-in of cell border in bleach ROI over time. Recovery of individual puncta is underscored by the dashed lines. (C) Fluorescence intensity plotted as a function of time. Mobile fraction = 20%. (D) Representative HaCaT cell transfected with Dsg2-mCherry, Gö6976 treated, and maintained in low-Ca²⁺ medium for 90 min. Scale bar = 5 µm. (E) Zoom-in of cell border in bleach ROI over time with individual puncta underscored by the dashed lines. (F) Fluorescence intensity plotted as a function of time. Mobile fraction = 7%.