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. 2020 May 12;219(6):e201906153. doi: 10.1083/jcb.201906153

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© 2020 Bartle et al.

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Figure S3. — Dsg3 order factor is not impacted by DP-S8249G-mCherry transfection. (A and B) Representative images of HaCaT cells cotransfected with (A) Dsg3-ΔEA-GFP and DP-wt-mCherry or (B) Dsg3-ΔEA-GFP and DP-S2849G-mCherry and maintained in normal-Ca²⁺ medium. Scale bar = 5 µm. (C) Order factor of Dsg3-ΔEA-GFP in cells expressing DP-wt-mCherry (n = 27) or DP S2849G-mCherry (n = 26; ns, not significant, P > 0.05; Student’s t test). (D) Quantification of adhesive strength by dispase fragmentation assay in HaCaT cells expressing either wt or mutant DP with Gö6976 and Ca²⁺ conditions indicated (n = 3; mean ± SD; **, P ≤ 0.01; Student’s t test).