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. 2020 May 12;219(6):e201906153. doi: 10.1083/jcb.201906153

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© 2020 Bartle et al.

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Figure S4. — Tagged proteins are expressed at the expected molecular weights. Cells were transiently transfected with plasmids as indicated. 48 h after transfection, cells were harvested for SDS-PAGE and Western blot analysis. Each blot includes an untransfected control. (A) Cos7 cells transfected with GFP, Dsg3-ΔEA-GFP, or Dsg3-ΔEA-W2A-GFP blot were probed for Dsg3 or GFP. Tubulin was used as a loading control. (B) Cos7 cells transfected with mCherry, DP-mCherry, or DP-S2849-mCherry. Blot was probed for DP or mCherry with tubulin as a loading control. (C) A431 cells transfected with E-cad-GFP. Blot was probed with GFP and tubulin. (D) A431 cells transfected with PG-mEmerald. Blot was probed with GFP, and tubulin was used as a loading control. (E) A431 cells transfected with Dsg2-mCherry. Blot was probed with mCherry, and GAPDH was used as a loading control.