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. 2020 May 7;219(6):e201907154. doi: 10.1083/jcb.201907154

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© 2020 Kopf et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 7. — Lfc regulates microtubule-mediated adhesion resolution. (a–d) Cell shape outlines of non-treated control cells migrating under agarose under adhesive (a) or non-adhesive (PEG-coated; b) conditions. Cell shape outlines of nocodazole-treated cells migrating under agarose under adhesive (c) or non-adhesive (PEG-coated; d) conditions. Upper panels show littermate control wild-type cells. Lower panels show Lfc^−/− cells. Scale bar, 10 µm. See also Video 10. (e) Cell lengths of non-treated control cells migrating under adhesive and non-adhesive conditions (n = minimum of 80 cells per condition from N = 5 experiments). Boxes extend from 25th to 75th percentiles. Whiskers span minimum to maximum values. Annotation above columns indicates results of Kruskal-Wallis with Dunn’s test; ****, P ≤ 0.0001. (f) Cell lengths of nocodazole-treated cells migrating under adhesive and non-adhesive conditions (n = minimum of 80 cells per condition from N = 5 experiments). Boxes extend from 25th to 75th percentiles. Whiskers span minimum to maximum values. Annotation above columns indicates results of Kruskal-Wallis with Dunn’s test; ****, P ≤ 0.0001. (g) Migration distance of Lfc^+/+ and Lfc^−/− DCs migrating under agarose under non-adhesive (PEG-coated) conditions of n = minimum of 80 cells per condition from N = 5 experiments. Cells were either non-treated or treated with nocodazole. Boxes extend from 25th to 75th percentiles. Whiskers span minimum to maximum values. Annotation above columns indicates results of one-way ANOVA; *, P ≤ 0.05; ****, P ≤ 0.0001. (h) Persistence of Lfc^+/+ and Lfc^−/− DCs migrating under agarose under non-adhesive conditions (PEG-coated). Cells were either non-treated or nocodazole-treated (n = minimum of 80 cells per condition from N = 5 experiments). Boxes extend from 25th to 75th percentiles. Whiskers span minimum to maximum values. Annotation above columns indicates results of Kruskal-Wallis with Dunn’s test; ****, P ≤ 0.0001. non-adhes., non-adhesive; n.s., not significant.