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Local MT depolymerization causes retraction. TAMRA-stained DCs migrating under agarose were recorded every 2 s on an inverted spinning-disk microscope and locally photoactivated (red boxes) every 40 s using a 405-nm laser line. Cells were either untreated (left panel) or treated with the photo-switchable MT depolymerizing agent PST-1 (right panel). Time in [min:s]. Scale bar, 10 µm. Frame rate, 10 frames per second.
