Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 7;219(6):e201907067. doi: 10.1083/jcb.201907067

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Liu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — HLH-30/TFEB drives longevity from combined inhibition of mitochondrial translation and dynamics. (A and B) Lifespan analysis performed in eat-3(tm1107) (A) and drp-1(tm1108) (B) subjected to RNAi against mrps-5 and hlh-30, individually or in combination. hlh-30 RNAi abolishes mrps-5 RNAi-mediated lifespan increase in both the eat-3(tm1107) and the drp-1(tm1108) mutant worms. See Table S1 for lifespan statistics. (C) Representative micrographs of worms expressing HLH-30::GFP grown on ev, mrps-5 RNAi, eat-3 RNAi, or mrps-5;eat-3 double RNAi bacteria (as indicated). Images were taken at day 2 of adulthood. χ² tests were performed to assess the significance of differences in the levels of HLH-30::GFP nuclear enrichment among RNAi-treated N2 worms (P = 0.0001, P = 0.0005, and P = 0 when comparing mrps-5 RNAi + ev, eat-3 RNAi + ev, and mrps-5 + eat-3 RNAi to ev-treated controls, respectively). The upper panels show fluorescent images of HLH-30::GFP nuclear localization, while the lower panels display overlay of HLH-30::GFP fluorescent images with bright field images of worms. Scale bar in ev-treated condition is 100 µm and applies to all the images in C. White arrowheads indicate intestinal nuclei. (D) Quantification of HLH-30 nuclear localization in C: n = 16–29 different animals per RNAi treatment, pooled from two independent experiments. (E) HLH-30::GFP nuclear localization in intestinal cells of wild-type, drp-1(tm1108) mutant, and drp-1;fzo-1 double mutant worms at day 2 of adulthood. Scale bar in ev-treated N2 is 100 µm and applies to all the images in E. White arrowheads indicate intestinal nuclei. (F) Quantification of HLH-30 nuclear enrichment in E: n = 21–32 different animals per RNAi treatment, pooled from two independent experiments. χ² tests were performed to assess the significance of differences in the levels of HLH-30::GFP nuclear enrichment in N2, drp-1(tm1108) mutant, and drp-1;fzo-1 mutant upon mrps-5 RNAi (P = 0, P = 0.0314, and P = 0.5533 when comparing mrps-5 RNAi to ev-treated controls in N2, drp-1(tm1108), and drp-1;fzo-1, respectively).