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. 2020 May 20;219(6):e201702151. doi: 10.1083/jcb.201702151

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© 2020 Gebhardt et al.

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Figure S1. — Wide-field Fura-2 microfluorimetry data from freshly dissociated mouse DRG neurons. In the absence of external calcium, we do not observe calcium transients in neurons stimulated by brief (30-s) applications of the lysosome disruptor GPN (200 µM) using 1 mM (middle panel) or 10 mM (lower panel) EGTA as external calcium buffer. In the presence of external calcium (upper panel), 3 of 244 neurons showed small and variable responses. These findings are in contrast to small cytosolic calcium transients evoked by 5-min application of GPN (Fig. 3 I), which paralleled the disruption of lysosomes (Fig. 3 G) with a mean time to peak calcium increase of 100 s.