Table 1. List of proteins identified by MS after subcellular fractionation and K48-Ub immunoprecipitation using NEs as starting material.
| Quantitative value (normalized total spectra) | Total unique peptide count | Percent coverage (%) | ||||
|---|---|---|---|---|---|---|
| Protein name | 4TorKO IP | WT IP | 4TorKO IP | WT IP | 4TorKO IP | WT IP |
| UBA52 | 49.16 | 35.03 | 11 | 11 | 50.00 | 50.00 |
| ACTC1 | 33.09 | 27.60 | 25 | 16 | 47.20 | 44.30 |
| HSPA1A | 29.30 | 7.43 | 23 | 6 | 36.20 | 11.90 |
| MLF2 | 7.56 | 2.12 | 8 | 2 | 35.90 | 12.10 |
| HSPA8 | 48.21 | 25.48 | 27 | 20 | 34.80 | 33.60 |
| VCP | 29.30 | 31.84 | 26 | 29 | 34.60 | 38.20 |
| Emerin | 8.51 | 1.06 | 8 | 1 | 33.50 | 4.72 |
| UBAC2 | 8.51 | 3.18 | 8 | 3 | 29.90 | 13.70 |
| DNAJB2 | 8.51 | 1.06 | 6 | 1 | 27.80 | 3.09 |
| DNAJB6 | 7.56 | 2.12 | 5 | 2 | 23.60 | 8.97 |
| ERLIN2 | 7.56 | 11.68 | 6 | 6 | 22.40 | 22.40 |
| ACTBL2 | 8.51 | 13.80 | 7 | 8 | 20.50 | 26.30 |
| SARAF | 8.51 | 0.00 | 5 | 0 | 19.20 | 0.00 |
| VDAC1 | 5.67 | 9.55 | 5 | 7 | 18.70 | 33.60 |
| APOA1 | 3.78 | 10.61 | 4 | 9 | 18.70 | 34.50 |
| HSPA9 | 10.40 | 8.49 | 9 | 8 | 18.00 | 16.20 |
| CANX | 6.62 | 11.68 | 7 | 7 | 15.50 | 16.20 |
| DNAJB12 | 5.67 | 0.00 | 5 | 0 | 13.60 | 0.00 |
| PDE12 | 5.67 | 3.18 | 6 | 3 | 13.50 | 6.24 |
| KCNG1 | 9.45 | 0.00 | 7 | 0 | 10.50 | 0.00 |
Proteins shown are the top 20 candidate proteins scored by highest percent sequence coverage in 4TorKO cell IP.