Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 9;219(6):e201910019. doi: 10.1083/jcb.201910019

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S1. — Acetylation tubulin as a marker for centriole length. (A) RPE-1 cells were expanded ∼4.2× and labeled for acetylated tubulin (ac tub) and β tubulin. Left: Examples of centrioles in various cell cycle stages. Presented centrioles were selected for brighter and more even β tubulin signal, as β tubulin antibody, in our hands, does not evenly label all centrioles in the population. Right: The comparison of mother centriole length determined by measuring the length of acetylated and β tubulin signal. Acetylated tubulin is a reliable marker for the analysis of the length of mature centrioles. (B) The pattern of centriole acetylation during cell cycle progression. Cycling HeLa cells were expanded ∼4.2× and immunolabeled for acetylated tubulin and distal centriole protein Cep290. Four mother centrioles (MC) associated with procentrioles (PC) from different phases of the cell cycle are shown. The intensity plots of the fluorescent acetylated tubulin (green) and Cep290 signal (magenta) along red and yellow arrows are shown below. MC acetylated tubulin signal is present along microtubule walls and overlaps with Cep290 signal on centriole’s distal ends. Cep290 accumulates to procentriole distal ends from their early stages. Elongating procentrioles in mitosis and immediately after mitosis show a gradient of acetylated tubulin signal from proximal toward distal end. Due to such a gradient, the length of PCs, if determined by measuring only the length of acetylated tubulin signal, may be in some cells slightly underestimated. Scale bars, 2 µm.