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. 2020 May 15;219(6):e201912037. doi: 10.1083/jcb.201912037

Figure S3.

Figure S3.

MEI-1 phosphorylation (S92) is necessary and sufficient to target MEI-1 for degradation. Western blot analysis of embryonic extracts from N2 (WT control, ctrl), FLAG::MEI-1 WT (light blue), FLAG::MEI-1 S92A (orange), and FLAG::MEI-1 S92D (green), animals exposed to mock (control) or mei-1(RNAi) using anti-MEI-1 (upper panel) and anti-tubulin (middle panel, loading control) antibodies. The lower panel shows Ponceau staining of the membrane.