| Antibodies |
|
|
| Rabbit anti-MASTL (1:1,000 for Western blot [WB]) |
Sigma-Aldrich |
Cat# HPA027175; RRID:AB_1853591 |
| Rabbit anti-MASTL (1:1,000 for WB) |
Abcam |
Cat# ab86387; RRID:AB_1925198 |
| Mouse anti-α-tubulin (1:1,000 for WB, 1:100 for immunofluorescence [IF]) |
Hybridoma bank |
12g10anti-alpha-tubulin |
| Mouse anti-β1-integrin CD29 (1:1,000 for WB) |
BD Biosciences |
Cat# 610468; RRID:AB_397840 |
| Mouse anti-GAPDH (1:20,000 for WB) |
HyTest |
Cat# 5G4MAB6C5 |
| Mouse anti-paxillin (1:100 for IF, 1:1,000 for WB) |
BD Biosciences |
Cat# 612405, RRID:AB_647289 |
| Rabbit anti-phospho-MLC 2 (Thr18/Ser19; 1:100 for IF) |
Cell Signaling Technology |
Cat# 3674, RRID:AB_2147464 |
| Rabbit anti-MLC (phospho S20; 1:500 for WB) |
Abcam |
Cat# ab2480, RRID:AB_303094 |
| Rabbit anti-MLC 2 (1:500 for WB) |
Cell Signaling Technology |
Cat# 3672, RRID:AB_10692513 |
| Rabbit anti-GEF-H1 (1:1,000 for WB) |
Cell Signaling Technology |
Cat# 4076, RRID:AB_2060032 |
| Rabbit anti-phospho-GEF-H1 (Ser886; 1:1,000 for WB) |
Cell Signaling Technology |
Cat# 14143, RRID:AB_2798402 |
| Rabbit anti-tropomyosin 4.2 (Delta Tm 9d; 1:1,000 for WB, 1:100 for IF) |
A kind gift from Prof. Peter Gunning (UNSW Sydney, Sydney, Australia) |
(Schevzov et al., 2011) |
| Mouse anti-nonmuscle myosin IIB (1:1,000 for WB) |
Abcam |
Cat# ab684, RRID:AB_305661 |
| Mouse anti-vinculin (1:100 for IF, 1:1,000 for WB) |
Sigma-Aldrich |
Cat# V9131, RRID:AB_477629 |
| Mouse anti-β-actin (clone AC-15; 1:1,000 for WB) |
Sigma-Aldrich |
Cat# A1978, RRID:AB_476692 |
| Recombinant anti-β-catenin antibody (E247; 1:100 for IF) |
Abcam |
Cat# ab32572, RRID:AB_725966 |
| Rabbit anti-E-cadherin (24E10; 1:100 for IF) |
Cell Signaling Technology |
Cat# 3195, RRID:AB_2291471 |
| Rabbit anti-GEF-H1 (IF 1:100) |
Abcam |
Cat# ab155785, RRID:AB_2818944 |
| Rabbit anti-phospho-paxillin (Tyr118; 1:1,000 for WB) |
Cell Signaling Technology |
Cat# 2541, RRID:AB_2174466 |
| Mouse anti-MRTF-A (G-8; 1:200 for IF) |
Santa Cruz Biotechnology |
Cat# sc-390324 |
| Donkey anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 568 (1:400 for IF) |
Thermo Fisher Scientific |
Cat# A10037, RRID:AB_2534013 |
| Donkey anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (1:400 for IF) |
Invitrogen |
Cat# A-21202 |
| IRDye 680RD donkey anti-mouse IgG (1:5,000 for WB) |
LI-COR Biosciences |
Cat# 926-68072 |
| Donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (1:400 for IF) |
Invitrogen |
Cat# A-21206 |
| IRDye 800CW donkey anti-rabbit IgG (1:5,000 for WB) |
LI-COR Biosciences |
Cat# 926-32213 |
| siRNAs and primers |
| AllStars negative control siRNA |
Qiagen |
1027281 |
| Hs_MASTL_6 FlexiTube siRNA (target sequence 5′-ACGCCTTATTCTAGCAAATTA-3′) |
Qiagen |
SI02653014 |
| Hs_MASTL_7 FlexiTube siRNA (target sequence 5′-CAGGACAAGTGTTATCGCTTA-3′) |
Qiagen |
SI02653182 |
| Hs_MASTL_11 FlexiTube siRNA (target sequence 5′-CAGCCCTTAGATTCAGATAGA-3′) |
Qiagen |
SI04441066 |
| Hs_MKL1_7 FlexiTube siRNA (target sequence 5′-ATCACGTGTGATTGACATGTA-3′) |
Qiagen |
SI04172028 |
| MRTF-A (sense strand 5′-CCAUCAUUGUGGGCCAGGU-3′) |
Sigma-Aldrich |
PDSIRNA5D |
| Hs_SRF_5 FlexiTube siRNA (target sequence 5′-CAAGATGGAGTTCATCGACAA-3′) |
Qiagen |
SI02757622 |
| RT-PCR primer for ARHGEF2 |
Universal Probe Library (Roche) |
| Forward: 5′-TACCTGCGGCGAATTAAGAT-3′ |
| Reverse: 5′-AAACAGCCCGACCTTCTCTC-3′ |
| Probe: 22 |
| RT-PCR primer for GAPDH |
Universal Probe Library (Roche) |
| Forward: 5′-GCCCAATACGACCAAATCC-3′ |
| Reverse: 5′-AGCCACATCGCTCAGACA-3′ |
| Probe: 60 |
| RT-PCR primer for MYH10 |
Universal Probe Library (Roche) |
| Forward: 5′-GGAGGACACGCTGGACAC-3′ |
| Reverse: 5′-TCTGCCACTTCTTGTTCACG-3′ |
| Probe: 3 |
| RT-PCR primer for MASTL |
Universal Probe Library (Roche) |
| Forward: 5′-GTCTGCAAACAATGTCTACTTGGTA-3′ |
| Reverse: 5′-CTAGAGCCAGTGCTACTTCAGAAA-3′ |
| Probe: 76 |
| RT-PCR primer for TPM4 |
Universal Probe Library (Roche) |
| Forward: 5′-CGGTGAAACGCAAGATCC-3′ |
| Reverse: 5′-GGCCACATCACCTTCAGC-3′ |
| Probe: 24 |
| qPCR primer for TBP |
N/A |
N/A |
| Forward: 5′-TGCACAGGAGCCAAGAGTGAA-3′ |
| Reverse: 5′-CACATCACAGCTCCCCACCA-3′ |
| qPCR primer for SRF |
N/A |
N/A |
| Forward: 5′-CAGATCGGTATGGTGGTCGG-3′ |
| Reverse: 5′-GTCAGCGTGGACAGCTCATA-3′ |
| qPCR primer for FOS |
N/A |
N/A |
| Forward: 5′-CTCTCTTACTACCACTCACCCGC-3′ |
| Reverse: 5′-GGTCCGTGCAGAAGTCCTGCG-3′ |
| qPCR primer for VCL |
N/A |
N/A |
| Forward: 5′-ACCAAGGCATAGAGGAAGCTTTA-3′ |
| Reverse: 5′-GCTCTCTTCATGGCTTCAGTG-3′ |
| DNA constructs |
| pcDNA EGFP-C2 |
This paper |
N/A |
| pcDNA EGFP MASTL WT (siRNA resistant) |
This paper |
N/A |
| pcDNA EGFP MASTL G44S (siRNA resistant) |
This paper |
N/A |
| pcDNA EGFP MASTL E167D (siRNA resistant) |
This paper |
N/A |
| pIND20-MRTF-A-GFP |
Hinojosa et al., 2017 |
N/A |
| mEmerald-paxillin-22 |
A kind gift from Prof. Robert Grosse, Institute of Experimental and Clinical Pharmacology and Toxicology, University of Freiburg and CIBSS – Center for Integrative Biological Signalling Studies, Freiburg, Germany (plasmid 54219; Addgene; http://n2t.net/addgene:54219; RRID:Addgene_54219) |
(Paszek et al., 2012) |
| pcDNA3.1 EGFP ARHGEF2 |
A kind gift from Prof. Michael Sixt (The Institute of Science and Technology Austria, Klosterneuburg, Austria) |
Kopf et al., 2019 Preprint
|
