. 2020 May 21;219(6):e201905085. doi: 10.1083/jcb.201905085

Table 1. List of oligos.

Name	Sequence (5′- to -3′)	Purpose	Construct
EH-524	`gagctctcgagaattctcacgcgtatgcagatcccacaggcg`	Clone human PD-1^{WT or FF} into pHR-mGFP vector	pHR-PD-1^{WT or FF}-mGFP
EH-863	`gcaagcttgatatcctgcagacgcgtcaggggccaagagcagt`	Clone human PD-1^{WT or FF} into pHR-mGFP vector	pHR-PD-1^{WT or FF}-mGFP
EH-741	`gagctctcgagaattctcatgaagacattgcctgccat`	Clone human BTLA^{WT or FF} into pHR-mGFP vector	pHR-BTLA^{WT or FF}-mGFP
EH-742	`caagcttgatatcctgcagacgcgtactcctcacacatatggatg`	Clone human BTLA^{WT or FF} into pHR-mGFP vector	pHR-BTLA^{WT or FF}-mGFP
EH-1004	`ctggccgtcatctgctccagaaggcaccaaggaaagc`	Pair with EH-742, clone human BTLA^INT into pHR-mGFP vector	pHR–PD-1^ECTO-TMD–BTLA^INT-mGFP
EH-1005	`ggagcagatgacggccag`	Pair with EH-524, clone human PD-1^ECTO-TMD into pHR-mGFP vector	pHR–PD-1^ECTO-TMD–BTLA^INT-mGFP
EH-1006	`ctgcctgttctgctgcctgcgggccgcacgagggaca`	Pair with EH-863, clone human PD-1^INT into pHR-mGFP vector	pHR–BTLA^ECTO-TMD–PD-1^INT–mGFP
EH-1007	`caggcagcagaacaggcag`	Pair with EH-741, clone human BTLA^ECTO-TMD into pHR-mGFP vector	pHR–BTLA^ECTO-TMD–PD-1^INT–mGFP
EH-1829	`gagctctcgagaattctcacgcgtatgaggatatttgctgtctttatattcatg`	Clone human PD-L1 into pHR-mCherry vector	pHR–PD-L1–mCherry
EH-959	`caccatggtggcgaccggtggatccgtctcctccaaatgtgtatc`	Clone human PD-L1 into pHR-mCherry vector	pHR–PD-L1–mCherry
EH-70	`agctctcgagaattctcatggagcctcctggag`	Clone human PD-L1 into pHR-mRuby vector	pHR-HVEM-mRuby
EH-71	`caagcttgatatcctgcagacggtggtttgggctcctccc`	Clone human PD-L1 into pHR-mRuby vector	pHR-HVEM-mRuby
EH-1391	`tggaggctcgagcggtggcggccgcatggtgaggtggtttcaccg`	Clone human SHP1 into pHR-mCherry vector	pHR-mCherry-SHP1
EH-1392	`cgactctagagtcgcggccgctcacttcctcttgagggaacc`	Clone human SHP1 into pHR-mCherry vector	pHR-mCherry-SHP1
EH-1078	`tggaggctcgagcggtggcggccgcatgacatcgcggagatgg`	Clone human SHP2 into pHR-mCherry vector	pHR-mCherry-SHP2
EH-1079	`tctagagtcgcggccgcttcatctgaaacttttctgctgttg`	Clone human SHP2 into pHR-mCherry vector	pHR-mCherry-SHP2
EH-396	`tctgttccaggggcccctgggatcccgggccgcacgagg`	Clone human PD-1^{INT (WT or FF)} into pGEX6p2-GST vector	pGEX6p2-GST–PD-1^{INT (WT or FF)}
EH-397	`ggccgctcgagtcgacccgggttacaggggccaagagc`	Clone human PD-1^{INT (WT or FF)} into pGEX6p2-GST vector	pGEX6p2-GST–PD-1^{INT (WT or FF)}
EH-637	`ttaactttaagaaggagatataccatgtcccctatactaggt`	Clone GST-human BTLA^{INT (WT or FFFF)} into pET28A-TwinStrep vector	pET28A-GST-BTLA^{INT (WT or FFFF)}-TwinStrep
EH-607	`tccacctttctcgaactgcgggtggctccaggatccactcctcacacatatgg`		pET28A-GST-BTLA^{INT (WT or FFFF)}-TwinStrep
EH-1070	`gagctctcgagaattctcacgcgtatgtgggtccggcag`	Clone mouse PD-1 into pMIG II-mGFP vector	pMIG II-mouse PD-1–mGFP
EH-1071	`agcttgatatcctgcagacgcgtaagaggccaagaacaatgtc`	Clone mouse PD-1 into pMIG II-mGFP vector	pMIG II-mouse PD-1–mGFP
EH-1376	`gagctctcgagaattctcacgcgtatgaagacagtgcctgccatg`	Clone mouse BTLA into pMIG II-mGFP vector	pMIG II-mouse BTLA-mGFP
EH-1377	`caagcttgatatcctgcagacgcgtacttctcacacaaatggatgc`	Clone mouse BTLA into pMIG II-mGFP vector	pMIG II-mouse BTLA-mGFP
EH-1386	`tggaggctcgagcggtggcggccgcatggtgaggtggtttcaccgggacc`	Clone mouse SHP1 into pMIG II-mCherry vector	pMIG II-mCherry-mouse SHP1
EH-1387	`tggaggctcgagcggtggcggccgcatgacatcgcggagatgg`	Clone mouse SHP1 into pMIG II-mCherry vector	pMIG II-mCherry-mouse SHP1
EH-1389	`cgactctagagtcgcggccgctcacttcctcttgagagaacctttg`	Clone mouse SHP2 into pMIG II-mCherry vector	pMIG II-mCherry-mouse SHP2
EH-1390	`cgactctagagtcgcggccgctcatctgaaactcctctgctg`	Clone mouse SHP2 into pMIG II-mCherry vector	pMIG II-mCherry-mouse SHP2
EH-899	`caccgtcggcccagtcgcaagaacc`	SHP1_gRNA1	pX330-GFP SHP1_gRNA1
EH-900	`aaacggttcttgcgactgggccga`	SHP1_gRNA1	pX330-GFP SHP1_gRNA1
EH-901	`caccggacacctcggcccttgagc`	SHP1_gRNA2	pX330-GFP SHP1_gRNA2
EH-902	`aaacgctcaagggccgaggtgtc`	SHP1_gRNA2	pX330-GFP SHP1_gRNA2
EH-387	`caccggagacttcacactttccgtt`	SHP2_gRNA1	pX330-GFP SHP2_gRNA1
EH-388	`aaacaacggaaagtgtgaagtctcc`	SHP2_gRNA1	pX330-GFP SHP2_gRNA1
EH-389	`caccgtacagtactacaactcaagc`	SHP2_gRNA2	pX330-GFP SHP2_gRNA2
EH-390	`aaacgcttgagttgtagtactgtac`	SHP2_gRNA2	pX330-GFP SHP2_gRNA2
EH-26	`tggacagcaaatgggtcgcggacgggccgcacgaggga`	Clone human PD-1^INT into pET28A-His10 vector	pET28A-His₁₀–PD-1^INT
EH-1949	`gagctcgaattcgtcacaggggccaagagc`	Clone human PD-1^INT into pET28A-His10 vector	pET28A-His₁₀–PD-1^INT
EH-1498	`cagcaaatgggtcgcggaggcttctgggaggagttt`	Clone human SHP1^PTPase into pET28A-His₁₀ vector	pET28A-His₁₀-SHP1^PTPase
EH-1499	`gacggagctcgaattcttaataggtgatgttcccgtact`	Clone human SHP1^PTPase into pET28A-His₁₀ vector	pET28A-His₁₀-SHP1^PTPase
EH-1502	`ggacagcaaatgggtcgcggatccggcttttgggaggaatttg`	Clone human SHP2^PTPase into pET28A-His₁₀ vector	pET28aA-His₁₀-SHP2^PTPase
EH-1538	`gtcgacggagctcgaattctcaatacttaatatttgtatattcgtgcc`	Clone human SHP2^PTPase into pET28A-His₁₀ vector	pET28aA-His₁₀-SHP2^PTPase
EH-606	`ctttaagaaggagatataccatgggcagcagccatcat`	Clone His₁₀-human BTLA^{INT (WT, YFFF, FYFF, FFYF, or FFFY)} into pET28A-TwinStrep vector	pET28A-His₁₀-BTLA^{INT (WT, YFFF, FYFF, FFYF or FFFY)}-TwinStrep
EH-607	`tccacctttctcgaactgcgggtggctccaggatccactcctcacacatatgg`